Histology platform

Short description

The histology platform provides histological preparation and analysis services for biological samples, at cost price. From tissue fixation, standard and special stainings to in situ visualization of proteins-of-interest (immunohistochemistry, immunofluorescence) or nucleic acids (in situ, chromogenic or fluorescent hybridization), the platform enables researchers who frequent it to train and work in semi-autonomy on their samples. A technical team provides support in designing the project upstream and advising the researcher throughout the process, up to optical microscopy observation if necessary. The platform includes a motorized epifluorescence microscope (Olympus BX63) as well as the CellSens Dimension image analysis software, and table microscopes, all equipped with cameras. Access to the facilities is done in agreement with the technical manager, according to internal rules and booking of the requested equipment.

Expertise - Life sciences

fixation of biological samples
decalcification of mineralized tissues (tooth, bone)
paraffin/OCT embedding
microtomy / cryotomy
routine stainings (H.E./S., Masson trichrome, cresyl violet, P.A.S.)
special stainings (Picrosirius red, Fontana, Luxol Fast Blue, Eriochrome C, Safranin O and Toluidin blue)
immunohistochemistry
development and optimization of immunolabelings
direct and indirect immunofluorescence (IF), single or double
in situ hybridization (RNAScope® technology) with or without multiplex IF
list of antibodies available on request (for pilot experiments-only)