Confocal laser scanning microscope Leica SP5

Applications

This classical confocal microscope allows multicolor imaging on fixed samples. It allows Z-scanning for 3D reconstructions. Time-lapse imaging is possible on living samples, but only for short periods of time (no incubator, no drift correction system).

Technical description

Inverted Leica DMI6000CS, with stage manual for XY and motorized for Z movements
Lasers: 405 nm (diode), 488 nm (Ar), 561 nm (diode), 633 nm (HeNe)
Detectors: 3 PMTs for fluorescence + 1 PMT for transmitted light
Objectives: 10x (PL Fluotar NA 0.3, dry), 40x (PL-Apo CS, NA 1.3, oil UV), 63x (PL-Apo CS, NA 1.40, oil UV), 100x (PL-Apo CS, NA 1.40, oil)
Drift correction: None
UV-Vis light source: Short Arc Lamp HXP Metal halide (HXP R120W/45C VIS)
Antivibration table (stone)